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CN109406659A 一种鉴定支气管扩张症生物标记物的方法及其检测试剂盒 无效 - 撤回

  • 专利标题： 一种鉴定支气管扩张症生物标记物的方法及其检测试剂盒
  • 专利标题（英）： Method and detection kit for identifying bronchiectasis biomarkers
  • 申请号： CN201811323551.6
    申请日： 2018-11-01
  • 公开(公告)号： CN109406659A
    公开(公告)日： 2019-03-01
  • 发明人: 张丽娟 , 郝翠 , 唐立岷
  • 申请人： 青岛大学附属医院
  • 申请人地址： 山东省青岛市市南区江苏路16号
  • 专利权人： 青岛大学附属医院
  • 当前专利权人： 青岛大学附属医院
  • 当前专利权人地址： 山东省青岛市市南区江苏路16号
  • 主分类号： G01N30/02
  • IPC分类号： G01N30/02 ;  G01N30/06
一种鉴定支气管扩张症生物标记物的方法及其检测试剂盒
摘要：
本发明提供了用于鉴定支气管扩张症的生物标记物及其应用。所述生物标记物为患者血清经过柱前1-苯基-5-甲基吡唑啉酮(PMP)衍生的高效液相色谱(HPLC)得到的游离葡萄糖和甘露糖。本发明的技术方案具有前处理简单，分析时间短，仪器价格合理，符合常规使用，操作步骤简单易学，检测结果准确性高，只需采血进行HPLC仪分析便可区分正常人和患者，并且所需血清量极少，采血量小于1mL等优点。所得结果显示此分析方法可以快速分析血清中游离的甘露糖和葡萄糖，并可以计算得到甘露糖、葡萄糖糖浓度及二者峰面积，对于研究血清游离甘露糖和葡萄糖与疾病之间的关系、寻找新型支气管扩张症疾病临床检测的标志物有着非常重要的意义。
摘要（英）：

The invention provides biomarkers used for identifying bronchiectasis and application thereof. The biomarkers are free glucose and free mannose which are obtained by subjecting patient serum to pre-column 3-methyl-1-phenyl-2-pyrazolin-5-one (PMP) derivatization high-performance liquid chromatography (HPLC). According to the technical scheme, the biomarkers used for identifying bronchiectasis havethe advantages that the pre-treatment is simple, the analysis time is short, the instrument price is reasonable, the conventional use is met, the operation steps are simple and easy to learn, the detection result is high in accuracy, a normal person and a patient can be distinguished by just sampling blood for HPLC instrument analysis, moreover, the required serum amount is very few, the amount ofsampled blood is less than 1 mL, and the like. An obtained result shows that by means of an analysis method, the free mannose and the free glucose in the serum can be fast analyzed, and the concentrations and peak areas of the mannose and the glucose can be calculated, so that the method and the detection kit for identifying bronchiectasis biomarkers have significant meaning in studying a relation between the free mannose and the free glucose in the serum and bronchiectasis and finding a novel bronchiectasis clinical detection marker.

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