The invention belongs to the agricultural biotechnical field and in particular relates to manganese peroxidase NfMnP, and an encoding gene and an application thereof. The invention provides the manganese peroxidase NfMnP originated from a lignocellulose degrading bacterium nematoloma frowardii. The amino acid sequence of the manganese peroxidase is as shown in SEQ ID No.1. The manganese peroxidasecan degrade mycotoxins of different structure types effectively. As a novel enzyme preparation, the manganese peroxidase NfMnP can be widely applied to the field of mycotoxin detoxification of food and feed.