The invention provides an adhesive and a preparation method thereof, and a use thereof in a lithium-sulfur battery. The adhesive comprises a mixed solution of an epoxidized natural rubber emulsion anda polyethyleneimine solution. The adhesive can be used as a binder for an anode of the lithium-sulfur battery. In a curing process, epoxidized natural rubber in the epoxidized natural rubber emulsionreacts with polyethyleneimine in the polyethyleneimine solution to form a three-dimensional network macromolecule. The macromolecule can adsorb and bind a polysulfide to prevent the polysulfide frombeing dissolved in electrolyte and further avoid a shuttle effect of the polysulfide to prevent the polysulfide from entering a cathode. Meanwhile, the three-dimensional network macromolecule is highin flexibility and can adapt to a volume change of sulfur in an electrical cycle through an elastic change of a molecular chain. In short, the use of the adhesive can effectively improve the problemsthat the polysulfide is easy to dissolve and shuttle, thereby effectively improving the problem of attenuation of the battery cycle performance and capacity of a sulfur-based active material during use as an active ingredient of an anode material of the lithium-sulfur battery.