The invention discloses a dendrobium officinale seedling tissue culture method which comprises the following steps: pre-degerming dendrobium officinale capsules; incising the treated dendrobium officinale capsules; uniformly scattering seeds in a germinating culture medium to culture to obtain protocorms; then transferring the protocorms to a proliferation medium to culture to obtain protocorm-like bodies; then transferring the protocorm-like bodies to a differentiating culture medium to culture to obtain seedlings; then transferring the seedlings to a tissue culture bottle containing a seedling culture medium to culture or strengthening the seedlings; hardening the seedlings by the tissue culture bottle containing the strong seedlings; at the beginning of seedling hardening, adding 60-70mL mineral water per sq.m; in three weeks of seedling hardening, adding 20-30 mL nutritional liquid per sq.m; then adding 10-18 mL mineral water; and then, adding the nutritional liquid once every 5 days, and transplanting the seedlings after adding the nutritional solution for 2-3 times. The method can improve the transplanting survival rate of dendrobium officinale, the dendrobium officinale isstable in quality and scaled planting of dendrobium officinale is promoted favorably.