The invention relates to the field of traditional Chinese medicines and particularly provides application of a traditional Chinese medicinal composition in preparing drugs for preventing and/or treating atrophic vaginitis. The traditional Chinese medicinal composition provided by the invention is prepared from the following raw materials: rhizoma alismatis, semen plantaginis, herba patriniae, floslonicerae, cortex moutan, herba hedyotidis diffusae, radix paeoniae rubra, agrimonia pilosa, rhizoma coptidis and cortex phellodendri. The traditional Chinese medicinal composition can be used for preparing drugs for preventing and/or treating the atrophic vaginitis and has obvious curative effects. The traditional Chinese medicinal composition has significantly improvement on symptoms of increased pH value of vagina, decrease of estrogen in blood and urine, reduction of microecological diversity, reduction of bacterial gene abundance and the like; the probability that a body is suffered fromthe atrophic vaginitis can be effectively prevented; meanwhile, the treatment effect on the atrophic vaginitis is also obvious, the blank that the atrophic vaginitis especially senile vaginitis cannot be effectively treated is filled up and the abuse of antibiotics is avoided. The components of the traditional Chinese medicinal composition are all Chinese herbal medicines and can be repeatedly used for a long time.