The invention provides a nano-operation device, and relates to the processing and manufacturing technical fields. The nano-operation device includes a first motion mechanism, a second motion mechanismand a control device. The first motion mechanism has multiple translational degrees of freedom and at least one rotational degree of freedom. The second motion mechanism has multiple rotational degrees of freedom and at least one translational degree of freedom. The first motion mechanism and the second motion mechanism are each equipped with an actuating end. The first motion mechanism and the second motion mechanism are both connected with the control device; the control device adapts to control the first motion mechanism and the second motion mechanism to drive the motion of the actuatingends. The actuating ends are arranged on the first motion mechanism and the second motion mechanism, micro tweezers are formed with cooperative use of a plurality of the actuating ends, and nanowiresor nanoparticles are clamped.