The invention discloses a method for preparing xylitol and mixing syrup from xylose mother liquor. The method comprises the steps: pretreatment, specifically, a calcium hydroxide solution is added into the xylose mother liquor to adjust the pH to 8.5-9.0, carbon dioxide is introduced to adjust the pH to 6.6-7.0, and part of calcium ions are removed by cation exchange resin; chromatographic separation to obtain an extracting solution and raffinate; decolorization and filtration; hybridization and purification; evaporation, specifically, the extracting solution subjected to hybridization and purification is vacuum-concentrated till the mass percentage concentration of dry matter is 75-85%, and the raffinate is vacuum-concentrated till the mass percentage concentration of the dry matter is 65-75%, so that a finished mixing syrup product is obtained; crystallization; centrifugation and drying; dissolution and hydrogenation; and refining to obtain xylitol crystals. According to the invention, the xylose mother liquor is pretreated by calcium hydroxide and carbon dioxide; the method is simple and easy; the effect is obvious; impurities are removed; the viscosity of the xylose mother liquor is lowered; and the filtration speed is increased. All monosaccharide components of the xylose mother liquor are utilized, so that the additional value of the xylose mother liquor is increased.