The invention relates to a drought-resistant and salt-tolerant gene IpNY-B1 as well as an encoding protein and an application thereof. The gene IpNY-B1 is obtained by cloning from Ipomoea pes-caprae as an important wild plant in sand beach, and drought resistance and salt tolerance of plants can be improved by transferring the gene into cells, tissue or individuals of host plants; resistance of engineered strains to high salinity and oxidative stress can be improved by transferring the gene into saccharomyces cerevisiae; the gene has remarkable application prospects in the field of improvementof resistance of the plants and the engineered strains.