The invention discloses a method for beat-to-beat heart rates calculation, which is applied to the technical field of heart rate measurement. The method comprises the following steps of: locating positions of any two adjacent heartbeats in a heart rate signal, determining an interval length range comprising the two heartbeats according to the positions of the two heartbeats, finding the interval length with the maximum similarity value of the two heartbeats in the interval length range, and calculating the beat-to-beat heart rates according to the positions of the two heartbeats and the interval length with the maximum similarity value of the two heartbeats. The invention also discloses a device for beat-to-beat heart rates calculation, electronic equipment and a storage medium. Accordingto the method, the device, the electronic equipment and the storage medium, disclosed by the invention, the calculation accuracy of the beat-to-beat heart rates is increased.