The invention provides a grape seed dye microcapsule and preparation and application methods thereof. The preparation method of the grape seed dye microcapsule comprises the following steps of 1) diluting alkali-soluble acrylic resin with water, then adjusting the pH to 10-12, and mixing the alkali-soluble acrylic resin with water until the alkali-soluble acrylic resin is fully dissolved to obtainan acrylic resin aqueous solution; mixing talcum powder aqueous solution into the acrylic resin aqueous solution to obtain a mixed solution; 2) mixing grape seed dyes with water to obtain a grape seed dye aqueous solution, mixing the grape seed dye aqueous solution into the mixed solution, adjusting the pH to be acid and performing stirring to obtain a nano suspension; 3) centrifuging, filteringand drying the nano suspension to obtain the grape seed dye microcapsule. Under the condition of ensuring dyeing depth and color fastness, the grape seed dye microcapsule can well control release of the dyes; after dyeing is completed, unbonded dye molecules remained in dye bath and on the surface of dyed fabric can be very low, and dye liquor COD (chemical oxygen demand) can be small; the grape seed dye microcapsule without releasing the dyes can be filtered and recovered to meet the demands of clean production.