The invention discloses a pouring construction method of a pipe jacking construction working well. The method comprises the steps of excavating the working well layer by layer; after each layer of theworking well is excavated, building a pouring formwork on the well wall; pouring concrete on the well wall; after each layer of the well wall is molded and demolded, installing a staircase extendingdownwards spirally in the circumferential direction on the well wall; excavating a next layer of the working well; laying a concrete conveying pipe on the well wall of an excavated layer which is 2-3layers higher than the current excavated layer along the lower portion of the staircase after the number of the excavated layers of the working well exceeds 3, wherein the upper end of the concrete conveying pipe is connected with a concrete conveying pipe or a pump outlet positioned at the upper layer, and the lower end of the concrete conveying pipe is detachably connected with a pouring pipe tocarry out concrete pouring on the well wall or top and bottom of the current excavated layer until excavation reaches the designed depth; finally, conducting concrete pouring at the bottom of the working well. The method has the advantages that the pouring construction operation is simple and convenient, the concrete conveying pipes are convenient to arrange, the labor intensity is reduced, and the construction safety is improved.