The invention provides a Tibetan medicine composition for treating conjunctivitis and keratitis of animals. The Tibetan medicine composition is prepared from the following crude medicines in parts byweight of 4-12 parts of radix scutellariae, 4-12 parts of barberry roots, 4-12 parts of figwortflower picrorhiza rhizomes, 3-9 parts of common scouring rush herbs and 3-9 parts of Tibetan artemisiae capillaris. The invention also provides a preparation method of the Tibetan medicine composition. According to the Tibetan medicine composition and the preparation method disclosed by the invention, through Tibetan medicine theory and traditional medication experience, a clinical technical method is combined, the medicine effect of the Tibetan medicine composition for treating conjunctivitis and keratitis of animals is screened, the formula is reasonable, safe and effective, the raw materials are easy to obtain, the preparation technology is simple, and the operation is simple and convenient; and clinical application test verifies that the effect of the Tibetan medicine composition for treating the conjunctivitis and the keratitis of the animals is notable, and the Tibetan medicine composition has great promotion and application value in clinical medicines.