The invention discloses a human glioma cell line. The preservation number of the human glioma cell line in the China General Microbiological Culture Collection Center is CGMCC NO.16693, and the preservation date is: November 07, 2018. The human glioma cell line of the invention can be stably multi-passaged, has tumorigenicity, can be prepare into a tumor animal model, and can be used for researchand development of human glioma diagnostic reagents, screening of human glioma drugs, and in vitro-studying of human gliomas. Moreover, the present invention differentiates glioma stem cells into glioma cells so as to firstly reduce the number of hetero cells, and after the glioma stem cells are differentiated into the glioma cells, the growth rate is fast, the proliferative ability is strong, andthe characteristics of pathological tissues of brain glioma is retained.