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Invention Grant
CN109628383B 重编程培养基及重编程诱导多能干细胞的培养方法 有权

重编程培养基及重编程诱导多能干细胞的培养方法
Abstract:
本发明涉及一种重编程培养基及重编程诱导多能干细胞的培养方法，该重编程培养基由DMEM‑F12基础培养基和添加成分组成，所述添加成分包括60μg/mL‑180μg/mL的L‑抗坏血酸、5.3μmol/L‑74μmol/L的氢化可的松、3ng/mL‑89ng/mL的亚硒酸钠、8μmol/L‑23μmol/L的Optiferrin、0.5μmol/L‑7.4μmol/L的视黄醇乙酸酯、40ng/mL‑60ng/mL的植物源重组人碱性生长因子、8μg/mL‑12μg/mL的IGF、0.2μmol/L‑0.6μg/mL的A‑83、2μmol/L‑6μmol/L的CHIR99021以及100μmol/L‑450μmol/L的丁酸钠。使用该重编程培养基不仅可以提高临床使用的安全性，还可以提高成体细胞重编程为诱导多能干细胞的效率。
Abstract(English):

The invention relates to a reprogrammed culture medium and a culture method of a reprogrammed induced multipotential stem cell. The reprogrammed culture medium is composed of a DMEM-F12 basal culturemedium and additive components, wherein the additive components comprise 60-180 microgram/mL of L-ascorbic acid, 5.3-74 micromole/L of hydrocortisone, 3-89 nanogram/mL of sodium selenite, 8-23 micromole/L of Optiferrin, 0.5-7.4 micromole/L of retinyl acetate, 40-60 nanogram/mL plant-derived recombinant human alkaline growth factors, 8-12 microgram/mL of IGF, 0.2-0.6 microgram/mL A-83, 2-6 micromole/L of CHIR99021 and 100-450 micromole/L of sodium butyrate. The use of the reprogrammed culture medium not only can improve the safety of clinical use, but also can improve the efficiency of adult cell reprogramming to induce the multipotential stem cell.

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