利索-全球专利检索

  1. 登录
  2. 注册
发明公开
CN109628490A 一种预防犬瘟热的shRNA重组腺相关病毒 无效 - 驳回

一种预防犬瘟热的shRNA重组腺相关病毒
摘要：
本发明属于基因工程技术领域，具体涉及一种预防犬瘟热的shRNA重组腺相关病毒，具体的，本发明公开了一种预防犬瘟热的shRNA重组腺相关病毒，由AAV2/9‑N‑shRNA3质粒和辅助质粒共转染AAV‑293细胞后得到shRNA重组腺相关病毒；所述干扰片段序列的Top strand如SEQ ID NO.7所示，bottom strand如SEQ ID NO.8所示；所述AAV2/9‑N‑shRNA3质粒，由经BamHI和EcoRI双酶切的pHBAAV‑U6‑CMV‑ZsGreen载体与干扰片段基因连接、转化后构建而成，并研究AAV2/9‑N‑shRNA3对预防犬瘟热的效果。
摘要（英）：

The invention belongs to the technical field of genetic engineering, and particularly relates to an shRNA recombinant gland related virus capable of preventing canine distemper. The invention particularly discloses the shRNA recombinant gland related virus capable of preventing the canine distemper. The shRNA recombinant gland related virus is obtained after cotransfection is conducted on an AAV-293 cell by an AAV2/9-N-shRNA3 plasmid and an auxiliary plasmid; Top strand of an interfering segment sequence is as shown in SEQ ID NO.1, bott om strand is as shown in SEQ ID NO.8, the AAV2/9-N-shRNA3plasmid is formed by the construction which is conducted after a pHBAAV-U6-CMV-ZsGreen carrier on which double enzyme digestion performed by BamHI and EcoRI is conducted is connected with the interfering segment sequence, and the effect of canine distemper preventing by the AAV2/9-N-shRNA3 is study.

0/0