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发明公开
CN109628561A 郁金香基腐病菌LAMP可视化检测方法 无效 - 撤回

郁金香基腐病菌LAMP可视化检测方法
摘要：
本发明公开了一种检测郁金香基腐病菌的LAMP引物对及其检测方法，属于植物病原真菌检测领域，自行设计合成了1套引物(Fot‑F3、Fot‑B3、Fot‑FIP和Fot‑BIP)。优化反应体系及扩增程序，建立了一种特异性强、快速准确并且可视化的植物及植物产品中郁金香基腐病菌基因组DNA的检测方法，一个工作日内完成整个检测，用于对郁金香基腐病菌的LAMP可视化检测。
摘要（英）：

The invention discloses an LAMP primer pair for detecting tulip basal decay bacteria and a detection method of the LAMP primer pair, and belongs to the field of plant pathogenic fungus detection. A set of primers (Fot-F4, Fot-B3, Fot-FIP and Fot-BIP) is automatically designed and synthesized. A reaction system and an amplification process are optimized, and the method which is high in specificity,rapid and accurate and realizes visual detection on tulip basal decay bacterium gene set DNA in plants and plant products is established, the detection is completed within one work day, and the method is used for conducting LAMP visual detection on the tulip basal decay bacteria.

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