The invention discloses a novel alkali-resistant penetrating agent for leather. The penetrating agent is prepared from, by weight, 10-15 parts of alcobol ethoxylate, 3-8 parts of methyl triethoxysilane, 55-65 parts of deionized water, 15-20 portions of n-polyoxyethylated-n-octadecylamine, 1-3 parts of sophorolipid, 8-10 parts of alkylphenol ethoxylates, 3-6 parts of stearic acid triethanolamine ester quaternary, 2-6 parts of sodium xylene sulfonate, 2-6 parts of trioctyl phosphate, 10-20 parts of sulfonated aliphatic polyester, 3-6 parts of acrylic acid and 5-8 parts of sucrose ester. The novel alkali-resistant penetrating agent for the leather is not only high in permeability and alkalinity but also effectively improves the comprehensive performance of the penetrating agent, and the penetrating performance of a leather dye can be obviously improved; the penetrating agent is economical and practical, the cost is low, damage of the leather can be effectively prevented, the service cycleis long, and the leather is not damaged.