The invention discloses a method for detecting a content of disperse dyes. The method for detecting the content of the disperse dyes comprises the following steps of pretreating a sample and detectinginstruments, wherein the pretreatment of the sample is weighing the sample, adding methanol-water extract liquid, wherein volume fraction of methanol is 40-60%, performing ultrasonic extraction at 60-70 DEG C for 30-45 min, filtering to obtain filtrate, and spin-drying the filtrate through a vacuum rotary evaporator; and adding methanol to meter volume, filtering by using a 0.22 micron filter membrane, and conducting high performance liquid chromatography-tandem mass spectrometry to detect and analyze the filtered solution with metered volume. According to the method for detecting the contentof the disperse dyes, solubility of the disperse dyes is taken into consideration in the pretreatment of the sample, a range of the disperse dyes is enlarged, namely, 30 types of disperse dyes are detected at the same time, operation is simple and convenient, analysis time is short, the separation effect is good, detection limits are low, an external standard method is accurate in qualitative andquantitative results, the detection limits and quantitative limit values of the 30 types of the disperse dyes are relatively low and located within 1-4 microgramme/L and within 3-12.5 microgramme/L correspondingly, and detection requirements of OEKO-TEX 100 2018 and GB/T 18885-2009. The standard recovery rate is within the range of 90.6-108.9 %, and precision value of the experiment is 6.8 % or less.