The invention provides a cypress close-to-nature medium container-grown seedling breeding and forest regeneration promoting method. According to the cypress close-to-nature medium container-grown seedling breeding and forest regeneration promoting method, an applied seedling medium is composed of forest subsoil and surface humus soil and filled into non-woven fabric bags or paper cups with the forest subsoil arranged under the surface humus soil, and then seed soaking, germination accelerating and sowing are timely performed; when growing out 4-6 pairs of primary leaves, seedlings are transplanted in rainy days in July to August and are then thin and after surviving and living through the winter; by breeding the seedlings in forest collecting ditches, forest nurseries built nearby ponds orindoor containers, seeds can be supplied with sufficient water, so that the emergence rate and the planting survival rate can be improved, the seedling breeding cycle can be short, and the size and the weight of transplanted seedlings can be small; meanwhile, due to the fact of being transplanted in rainy day in rainy seasons, the seedlings can be high in activity and overwintering rate. Besides,the cypress close-to-nature medium container-grown seedling breeding and forest regeneration promoting method lowers the demands on a large amount of labor, materials and costs during forest updatingwith 2-3-year-oil large seedlings higher than 1 meters, saves time, trouble, labor and cost and improves the cypress forest updating efficiency of limestone mountains.