The invention discloses a preparation method of a metal oxide and carbon nanotube compounded micro-column energy storage electrode. The preparation method belongs to the field of micro energy storagedevices. The micro-column energy storage electrode is prepared by the steps of: sequentially attaching Si/SiO2, micrograss and micro-column array structures onto a silicon substrate, then depositing an oxide and carbon nanotube compounded active thin film and current collectors serving as energy storage electrodes of the micro-column structures on the surfaces of the three-dimensional micrograss and the micro-column array structures, thereby combining the metal oxide and carbon nanotube compounded micro-column energy storage electrode. According to the preparation method, the ruthenium oxide,manganese oxide and the like are used as the metal oxides, and the micro-column and the ''micrograss'' structures are processed firstly; the ''micrograss'' structure is used as a deposition template,and a ''twisted'' corrugated three-dimensional spatial network is formed on the surface of the three-dimensional electrode by adopting the co-deposition process, the carbon nanotubes and the metal oxides are intertwined and have a large specific surface area. The prepared metal oxide and carbon nanotube compounded micro-column energy storage electrode has good energy storage capacity, has a specific capacity up to 272 mF/cm<2>, and has broad application potential.