The invention relates to circRNA and application thereof in preparation of a cervical cancer diagnostic reagent, in particular to application of hsa_circ_0000069 in preparation of a cervical cancer diagnostic reagent. Further mining and integrated analysis are carried out on sequencing data of cervical cancer, a biological information regulation network of the interaction of circRNA-miRNA-mRNA iscreated by means of a biological information method, and the key molecules hsa_circ_0000069 and hsa-miR-125b-5p which are involved in regulation of cervical cancer are searched for. A new molecular diagnostic marker for diagnosis of cervical cancer is provided, and a research basis for gene detection of clinical diseases is offered.