The invention discloses a composition capable of alleviating physical fatigues and a preparation method of the composition. Raw materials comprise a combination of rice bran fatty alkanol and at leastone of L-arginine or cortex cinnamomi, and further can comprise 15 to 20 parts of the rice bran fatty alkanol, 2 to 5 parts of the L-arginine, 1 to 3 parts of the cortex cinnamomi, 1 to 5 parts of raspberry fruit, 5 to 10 parts of oyster peptide, 5 to 10 parts of sea cucumber peptide, 5 to 10 parts of ocean fish oligopeptide, 1 to 3 parts of taurine, 1 to 5 parts of black ginger, 0.5 to 2 parts of conjugated linoleic acid and 0.2 to 0.6 part of yeast powder. The composition disclosed by the invention has a remarkable effect of alleviating the fatigues and especially has a remarkable effect onchronic fatigues.