The invention discloses a traditional Chinese medicine composition for treating prostate cancer, a preparation method thereof and a medicine thereof, and aims to provide a composition which has the effects of strengthening body resistance, resisting cancer, inducing diuresis and alleviating swelling, nourishing qi and yin, nourishing and strengthening bodies, prolonging life and the like, and canenhance the immunity of the patients with prostate cancer, inhibit the proliferation of prostate cancer cells, induce apoptosis, arrest cell cycle progression, inhibit cancer cell migration and vascular endothelial factor formation, alleviate the side effects of chemotherapeutic drugs and improve quality of life of the patients. The composition is characterized by comprising 15-25 parts by weightof phellinus igniarius growing on mulberry trees, 15-25 parts by weight of ganoderma leucocontextum, 15-25 parts by weight of ganoderma capense, 5-15 parts by weight of inonotus cuticularis, 5-15 parts by weight of trametes orientalis, 5-15 parts by weight of poria cocos, 5-15 parts by weight of pomitopsis officinalis fruiting bodies, 5-15 parts by weight of polyporus, and 5-15 parts by weight ofganoderma lucidum karst dried fruiting bodies, and belongs to the field of medical technology.