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发明公开
CN109679973A DNA聚合酶及其制备方法、表达基因、表达载体、宿主细胞和试剂盒 无效 - 驳回

DNA聚合酶及其制备方法、表达基因、表达载体、宿主细胞和试剂盒
摘要：
本发明涉及一种DNA聚合酶及其制备方法、表达基因、表达载体、宿主细胞和试剂盒。该DNA聚合酶的表达基因包括编码角质酶的核苷酸序列与编码角质酶的核苷酸序列连接的编码DNA聚合酶的核苷酸序列，上述DNA聚合酶的表达基因能够在宿主细胞中同时独立表达角质酶和DNA聚合酶。在角质酶的作用下，宿主细胞中表达的DNA聚合酶能够游离到宿主细胞外，使得采用上述DNA聚合酶的表达基因制备得到的DNA聚合酶中不含宿主细胞的DNA。
摘要（英）：

The invention relates to a DNA polymerase, a preparation method thereof, an expression gene, an expression vector, a host cell and a kit. The expression gene of DNA polymerase includes a nucleotide sequence of encoded cutinase and a nucleotide sequence of encoded DNA polymerase connected with the nucleotide sequence of encoded cutinase; the expression gene of the DNA polymerase is capable of simultaneously independent expressing cutinase and DNA polymerase in the host cell; under the action of cutinase, DNA polymerase expressed in the host cell is capable of drifting out of the host cell, so that the DNA polymerase prepared by adopting the expression gene of DNA polymerase contains no DNA of host cell.

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