The invention discloses a hot-melting adhesive antibacterial monofilament special for seamless hot pressing clothes and a preparation method of the hot-melting adhesive antibacterial monofilament. Thepreparation method comprises the following steps: adding a high-efficiency antibacterial agent into a pretreated polymer raw material; melting and composite spinning, and cooling and drafting, thereby obtaining the hot-melting adhesive antibacterial monofilament with a nonlinear shape special for composite seamless hot pressing clothes. According to the hot-melting adhesive antibacterial monofilament with the nonlinear shape special for the composite seamless hot pressing clothes, by utilizing the high efficiency of the antibacterial agent, by adding a certain small amount of metal ion antibacterial agent, the antibacterial monofilament can achieve broad spectrum antibacterial effects. Meanwhile, the antibacterial agent is difficult to separate out in the formation process, and the seamless hot pressing clothes prepared from the antibacterial monofilament have excellent antibacterial property and excellent fastness to washing.