The invention discloses a timing control method, a timing controller and a display device. The timing control method includes the following steps: judging whether a polarity reversal signal is reversed or not; if the signal is reversed, judging whether the polarities of two frames of image data before and after the reversal are the same; if the polarities are the same, detecting whether the firstgray scale difference of the two frames of image data meets a preset gray scale range; and if the preset gray scale range is met, when the polarity reversal signal is reversed, outputting a control signal by the timing controller to convert the control mode of charge sharing of a source driver from following the polarity reversal signal to following a line synchronization time signal, and/or to convert the gating time of the line synchronization time signal from the first time to the second time to further adjust the charging time of a data line. According to the embodiment provided by the invention, the problem of poor display effect caused by the reversal of the polarity reversal signal of a large-size display device can be solved, and the display performance of the display device can beeffectively improved.