The invention discloses a frame sealing adhesive mask, a manufacturing method of the frame sealing adhesive mask, a frame sealing adhesive manufacturing method and a display panel. The problems that in the prior art, the thicknesses, in the movement direction of a scraper and the direction perpendicular to the movement direction of the scraper, of a frame sealing adhesive manufactured on a displaybase board are not even, thus a display device has a Newton ring, and the performance of the device is affected are solved. The frame sealing adhesive mask comprises a mask body and a plurality of hollowed-out areas which are arranged inside the mask body and correspond to frame sealing area of multiple display base boards one by one; each hollowed-out area comprises a first hollowed-out area body extending in the first direction and a second hollowed-out area body extending in the second direction; the first direction is the movement direction of the scraper when the frame sealing adhesive mask is used for scraping the frame sealing adhesive; the part, surrounding the first hollowed-out area bodies, of the mask body has a first thickness; the part, surrounding the second hollowed-out area bodies, of the mask body has a second thickness; and the first thickness is smaller than the second thickness.