The invention discloses a man-machine arrangement parameter matching method in a comfortable driving posture of a driver by considering an electromyographic signal. The man-machine arrangement parameter matching method comprises the following steps: 1, respectively adjusting the positions of a steering wheel, a seat and an accelerator pedal of a test vehicle, and determining multiple groups of different experimental positions; 2, respectively testing the driver at the experimental positions, selecting an active muscle group with a high muscle activation degree, and acquiring an electromyographic signal of a static driving posture; 3, establishing a relation function between the muscle activation degree and discomfort after the muscle activation degree is determined by the electromyographicsignal, analyzing the weight of influence of the muscles on the discomfort, and calculating the overall discomfort of the static driving posture corresponding to the different experimental positions;and 4, determining the experimental position with minimal overall discomfort as the man-machine arrangement parameter.