The invention provides a tooth desensitizer product containing regenerative medicine materials and a preparation method thereof. The tooth desensitizer product containing regenerative medicine materials is mainly prepared from the regenerative medicine materials, a substrate material, a dispersing agent, a gelatinizing agent and others; an anhydrous vacuum high-speed shearing dispersing process isadopted for preparation. The regenerative medicine materials of the tooth desensitizer product containing the regenerative medicine materials are used as main functional components, the effect of preventing and treating tooth hypersensitivity is realized, the environment of the oral cavity can be further improved, and a certain effect of preventing and treating a periodontal disease, dental ulcerand dental plaque dental caries is realized.