The invention relates to a traditional Chinese medicinal composition for detoxification, and a preparation method and application thereof. The composition comprises, by weight, 30-50 parts of tribulusterrestris, 20-40 parts of cocklebur fruits, 1-10 parts of white mulberries, 2-12 parts of nightshade seeds and 25-48 parts of yellow wine. The traditional Chinese medicinal composition for detoxification has the advantages that the tribulus terrestris and the cocklebur fruits interact with each other to achieve the effects of tonifying qi and blood, supporting body resistance and enhancing bodyimmunity, and body injuries caused by bacteria and viruses are prevented due to the immunity enhancing effect; the white mulberries and the nightshade seeds serve as minister drugs to promote blood circulation, remove stasis, stop bleeding and relieve swelling and pain; the traditional Chinese medicinal composition has the effects of clearing away heat and toxic materials, removing dampness, dredging collaterals, strengthening the body resistance to eliminate pathogenic factors, and promoting tissue regeneration; the whole formula has high compatibility, and the herbs complement one another toachieve the effects of supporting the body resistance, tonifying the qi and the blood, promoting blood circulation, removing the stasis, and clearing away the heat and the toxic materials; the traditional Chinese medicinal composition has the advantages of good effect and quick action.