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发明公开
CN109797227A 一种柑橘半穿刺线虫特异性PCR检测方法及应用 无效 - 驳回

一种柑橘半穿刺线虫特异性PCR检测方法及应用
摘要：
本发明为“一种柑橘半穿刺线虫特异性PCR检测方法及应用”，属植物线虫分子检测技术领域。本发明公开了一种基于柑橘半穿刺线虫核糖体DNA特异性序列设计的一对特异性引物Ts1-F和Ts1-R，上述引物能从柑橘半穿刺线虫DNA中扩增获得特异性的片段，从而实现对柑橘半穿刺线虫的快速分子检测。本发明建立了一种灵敏度高、特异性强且快速准确的柑橘半穿刺线虫检测体系，在柑橘半穿刺线虫病的早期诊断和田间监测预警等方面，具有很高的应用价值。
摘要（英）：

The invention relates a tylenchulus semipenetrans specific PCR detection method and application, belongs to the technical field of plant nematodes molecular detection and discloses a pair of specificprimers Ts1-F and Ts1-R designed on the basis of a tylenchulus semipenetrans ribosome DNA specified sequence. The primers can be amplified in tylenchulus semipenetrans DNA to obtain a specific segment, in this way, quick molecular detection of tylenchulus semipenetrans can be realized. A tylenchulus semipenetrans detection system which is high in sensitivity and specificity, quick and accurate isestablished, and the method is of high application value in the aspects of early diagnosis and field monitoring prewarning of tylenchulus semipenetrans.

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