The invention discloses a method for detecting folic acid metabolism related gene mutation through combination of overlap extension PCR and Sanger sequencing and primer combination for detecting folicacid metabolism related gene mutation. A nucleotide sequence of the primer combination is shown as SEQ ID NO.1 to 8, and the primer combination is utilized to establish the detection method disclosedby the invention; according to the method disclosed by the invention, the overlap extension PCR and the Sanger sequencing are combined to detect folic acid metabolism related gene locus single nucleotide polymorphism, only once PCR reaction and once sequencing reaction are needed in the whole process, PCR reaction times and sequencing reaction times are remarkably reduced, and 6 mutation sites related to folic acid metabolism of MTHFR and MTRR genes can be detected; the method can be further utilized to find new variation points in amplified fragments. As the detection technology is applied to detecting folic acid metabolism related gene locus single nucleotide polymorphism, important clinical significance is achieved; thus, not only are operation steps simplified and is detection cost reduced, but also the detection efficiency is improved; the method is worth of popularizing.