The invention discloses an intelligent unstacking machine capable of preventing bottle collision. The intelligent unstacking machine comprises a bottle grabbing mechanism, a stack fixing device and aseparating plate grasping mechanism; bottles to be unstacked in layers are positioned to the accurate gripping positions through the stack fixing device; separating plates on the bottles to be unstacked in layers are removed through the separating plate grasping mechanism, and the bottles to be unstacked in layers are taken away through the bottle grabbing mechanism in a whole-layer manner; the stack fixing device comprises a stack fixing frame; the central axis of the stack fixing frame is the same as the central axes of a plurality of pneumatic grippers; and the stack fixing frame can inwards contract or outwards expand. By means of the intelligent unstacking machine, whole-layer unstacking of empty bottles with bottle trays can be achieved, the bottles to be unstacked in layers can be positioned to the accurate grasping positions, and the bottle grabbing efficiency is improved; meanwhile, the separating plates with the bottle trays can be prevented from being lifted along with the empty bottles; and the separating plates and the trays can be automatically collected, the automation degree of the equipment is improved, the labor intensity is reduced, the production efficiency is improved, the production cost of an enterprise is reduced, the safety hazard is reduced, and safe production is ensured.