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CN109828006A 一种检测甲基化RNA的光电化学传感器及其检测方法 失效 - 权利终止

一种检测甲基化RNA的光电化学传感器及其检测方法
摘要：
本发明公开了一种检测甲基化RNA的光电化学传感器及其检测方法，所述光电化学生物传感器，包括：电极，依次修饰在电极表面的硫化钨、纳米金、巯基苯硼酸、m6A抗体、甲基化RNA，混合了UTP的poly(U)聚合酶和Ru(NH3)63+。本发明利用WS2良好的光电活性和生物兼容性，纳米金良好的生物兼容性和导电性，抗体对抗原的特异性识别性能，以及用Poly(U)聚合酶延长RNA序列和Ru(NH3)63+作为信号扩大单元，实现对甲基化RNA的高灵敏性和高特异性检测。本发明的检测方法简单，实现了仪器小型化，且易于操作，即可实现对甲基化RNA的检测。
摘要（英）：

The invention discloses a photoelectrochemical sensor for detecting methylated RNA and a detection method thereof. The photoelectrochemical sensor comprises: an electrode, and tungsten sulfide, nanogold, mercaptobenzoic acid, m6A antibody, methylated RNA, poly(U) polymerase mixed with UTP and Ru(NH3)63+ which are modified on the surface of the electrode in order. The photoelectrochemical sensor for detecting methylated RNA and the detection method thereof utilize the good photoelectric activity and biocompatibility of WS2, the good biocompatibility and conductivity of nano gold, the specific recognition performance of antibodies to antigens, employ the Poly(U) polymerase to prolong the RNA sequence and take the Ru(NH3)63+ as a signal amplification unit to achieve the high-sensitivity and high-specificity detection for methylated RNA. The detection method is simple, achieves the miniaturization of the instrument, is easy to operation and achieves the detection for the methylated RNA.

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