The invention discloses a preparation method and application of bovine bone activated carbon, and relates to the technical field of comprehensive utilization of domestic wastes and preparation of organic pollutant adsorbents. In the method, zinc chloride, potassium hydroxide and phosphoric acid are respectively used as activators, and bovine bone carbonized substance is activated to prepare the bovine bone activated carbon. Experiments show that when the bovine bone is carbonized for 2 hours at 400 DEG C, zinc chloride is used as an activating agent, and the bovine bone carbonized substance isactivated for 90 minutes at 400 DEG C, so that the adsorption effect of the prepared bovine bone activated carbon on methylene blue is the best; according to the method, 0.3 g of activated carbon prepared from zinc chloride is added into 100 mL of 100 mg/L methylene blue solution, and the removal rate reaches 100% after 4.5 h. In addition, through determination, comparison and analysis of surfacefunctional groups of the bovine bone activated carbon, the methylene blue adsorption capacity of the bovine bone activated carbon is not only related to pores of the bovine bone activated carbon, butalso related to surface functional groups of the bovine bone activated carbon.