The invention discloses a promoter for regulating expression of a gene in nonglandular trichomes and an application of the promoter and relates to the technical field of gene engineering. A nucleotidesequence of the promoter is shown in SEQ ID NO.1. The invention also provides a preparation method of the promoter, a vector containing the promoter and an expression cassette. The promoter providedby the invention can guide specific expression of the reporter gene in the nonglandular trichomes of plants and has great significance in genetic engineering breeding for expressing and producing metabolites by use of nonglandular trichome tissue of plants.