The invention discloses a method and a system for realizing HTTP request interaction in a positive and negative isolation device. The method comprises the following steps: the first file text conversion module can orderly write request content of an initiated hypertext transfer protocol HTTP request to a file in a management large area to obtain a first file; the second file text conversion modulecan write the response header of the response result into one file and writes the response result into a second file associated with the text name of the response result, The first file text conversion module can analyze the monitored first file and the second file to obtain a hypertext transfer protocol HTTP result associated with the first file and the second file and feed back the HTTP result,and the front-end server can receive the fed back HTTP result. By means of the mode, it can be achieved that logic of an original service system does not need to be changed, the database synchronization problem does not need to be considered, instructions can be issued in real time, and compared with a traditional method, more troubles are saved, and data are safer.