The invention discloses construction of a whole genome high-throughput cloning vector in the field of biomedicine. The construction is characterized by comprising the steps of constructing a reverse transcription virus vector containing hTERT and SV40LT; conducting transfecting on PT67 packaging cells; using g418 and PM for combined screening to obtain plasmid packaging PT67 cells; extracting a culture solution of the plasmid packaging PT67 cells, and conducting transfecting on sp2/0 cells; carrying out combined screening by using G418 and PM to obtain highly immortalized plasmid packaging sp2/0 cells; after an exogenous whole genome is implanted, using HAT, G418 and PM for combined screening to obtain a highly immortalized hybrid strain capable of being cryopreserved and subjected to whole genome amplification. The hybrid strain can be used for industrial preparation of rare disease whole genome and construction of an immortalized gene bank of the rare disease whole genome.