The invention relates to a harvesting method of bamboo fungi. Fungus eggs planted in greenhouses and/or forests and/or bamboo forests are harvested 24-72 hours before the bamboo fungi are fruited, andthe harvested fungus eggs are put in a nutrient solution of a culture room to be cultured for fruiting, wherein the temperature of the culture room is controlled to be 10-20 DEG C, and the relative humidity of the culture room is controlled to be 75%-95%. According to the technical scheme, advance harvesting of the bamboo fungi is completed, the harvested fungus eggs are put in the culture room with the optimum fruiting condition, and therefore the fruiting yield is greatly increased. Meanwhile, as thallus contamination under the natural environment and the uncontrollable weather or animal influence is avoided during indoor fruiting, the damage rate of fruiting is greatly reduced.