The invention discloses a method for separating immune cells and further performing induced differentiation on the immune cells to form EPCs (endothelial progenitor cells). The method comprises stepsas follows: (1), peripheral blood is acquired, mononuclear cell layer fluid is sucked and added to a centrifugal tube, repeated centrifugation is performed, a supernatant is completely sucked after last centrifugation, and an RPMI1640 culture solution is utilized for blending to reach required concentration being about 2*10<7>/ml according to a counting result; (2), culture is performed and unattached cells are removed; (3), an RPMI1640 culture solution containing 10% RPMI1640 fetal calf serum is utilized, M-CSF, IL-3 and Orychophragmus violaceus component D are added, final volume of each culture hole is 1 ml, final concentrations of the Orychophragmus violaceus component D are 5 ng/ml, 0.4 ng/ml and 0.1-0.3 ng/ml respectively, a reagent is added once every two days, and attached cells are primarily cultured for 8 days.