The invention relates to the technical field of agricultural planting and particularly relates to a production method of selenium-enriched tobacco leaves. The production method comprises the followingsteps: selecting a production area; cultivating through selenium-gathered green manure; cultivating the selenium-enriched tobacco leaves; baking the tobacco leaves; specifying the requirements of selenium content of the selenium-enriched tobacco leaves; carrying out an inspection method. The method provided by the invention is used for planting the selenium-enriched tobacco leaves and the selenium-enriched tobacco leaves with the selenium content in the tobacco leaves of 0.25mg/kg to 4.00mg/kg can be produced; when the selenium-enriched cigarettes are produced by the selenium-enriched tobaccoleaves, the safety of the cigarettes is remarkably improved; the toxicity of cigarette tar and the content of free radicals are reduced through the selenium-enriched tobacco leaves and the drop reaches 10 to 30 percent; meanwhile, the releasing amount of harmful components including CO, B[a]P, NNK, NNN, NAT, NAB, hydrocyanic acid, crotonic aldehyde, hydrocyanic acid, ammonia and the like in smokeis reduced by 5 to 15 percent, so that the method has a wide application prospect.