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发明公开
CN109913576A 一种用于检测水稻抗褐飞虱基因Bph14的引物对及其应用 无效 - 驳回

一种用于检测水稻抗褐飞虱基因Bph14的引物对及其应用
摘要：
本发明公开一种用于检测水稻抗褐飞虱基因Bph14的引物对及其应用，所述用于检测水稻抗褐飞虱基因Bph14的引物对为共显性标记引物B14InD，所述共显性标记引物B14InD为：正向引物序列：CTACTGATTGCAGATTGACG；反向引物序列：CACTTGGTGAACTTATTCCCTT。本发明采用所述用于检测水稻抗褐飞虱基因Bph14的引物对经PCR扩增得到Bph14特异性共显性分子标记，其定位的抗褐飞虱基因位置明确，通过检测Bph14基因的特异性共显性分子标记，即可用于水稻品种或品系的基因型检测，可预测水稻植株的褐飞虱抗性，有利于提高抗褐飞虱水稻的育种效率。
摘要（英）：

The invention discloses a primer pair for detecting a rice brown planthopper resistance gene Bph14 and an application thereof. The primer pair for detecting the rice brown planthopper resistance geneBph14 is a co-dominance marker primer B14InD. The co-dominance marker primer B14InD is as follows: a forward primer sequence: CTACTGATTGCAGATTGACG; and a reverse primer sequence: CACTTGGTGAACTTATTCCCTT. The primer pair for detecting the rice brown planthopper resistance gene Bph14 is used for performing PCR amplification to obtain a Bph14 specificity co-dominance molecular marker, and the brown planthopper resistance gene positioned by the Bph14 specificity co-dominance molecular marker is clear in position. Through detecting the specificity co-dominance molecular marker of the Bph14 gene, namely genetype detection applied to rice varieties or strains, brown planthopper resistance of a rice plant can be forecasted, and it is beneficial to improve seed breeding efficiency of brown planthopper resistance rice.

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