The invention discloses a testing device for a security chip operating system, which is constructed through Python language and comprises a PCSC communication interface layer, an algorithm library interface layer, a function instruction interface layer, a basic algorithm interface layer and a script writing and executing layer. And communication with the to-be-tested security chip is realized through the PCSC communication interface layer. And the algorithm library interface layer is used for packaging interfaces of various security algorithms applied by the to-be-tested security chip. And thefunction instruction interface layer is used for packaging a function instruction in an operating system of the to-be-tested security chip. And the basic algorithm interface layer is used for performing secondary combination and packaging on the interface of the algorithm library interface layer and newly adding an operation function. The script writing and executing layer is used for storing, executing and modifying test scripts, and the script writing and executing layer achieves testing by combining and calling the PCSC communication interface layer, the algorithm library interface layer,the function instruction interface layer and the basic algorithm interface layer. The testing device for the operating system of the security chip can effectively improve the writing and debugging speed of the testing script.