The invention discloses warehouse detection material sample management automation based on the Internet of Things technology, and the method comprises the steps: employing a unified platform system torecord sample detection materials through a warehouse-in and warehouse-out document registration system; allowing the recorded and generated tasks to be subjected to disk assembling shelving or warehouse-out and unloading operation, so that the shelving and unloading tasks are completed. Independent storage of the sample materials in the three-dimensional warehouse is realized, and the special storage types and storage positions of the detection materials are automatically recommended. The shelving and unloadingare connected with automatic equipment, and the equipment automatically places thedetected materials on and off a designated warehouse position according to instructions. Stock update is performed. And after the instruction is executed according to the automation equipment, the GCP system inventory is automatically updated. According to the invention, warehouse-in and warehouse-out of sample detection materials with blind sample numbers and effective automatic warehouse management of upper and lower racks of the materials can be realized.