The invention belongs to the technical field of enzyme immobilization, and particularly relates to a method for immobilizing carbonyl reductase. Specifically, the macroporous resin is placed in a carbonyl reductase solution for adsorption fixation, and the macroporous resin is sequentially immersed and activated by an alkaline solution, ethanol, or an acidic solution. The immobilization method issimple in operation, small in enzyme loss and high in stability, and is suitable for industrial production such as mechanical stirring or fluidized bed.