The invention discloses a method for detecting tylosin residues in tissues, and belongs to the field of veterinary drug residue detection. The method comprises the following steps: preparing referencesubstance solution, preparing test sample solution, injecting the standard substance solution and the test sample solution into a liquid chromatograph in a gradient elution manner by adopting a highperformance liquid chromatography, and calculating tylosin residues according to an ultraviolet method. The detection method of tylosin residues in tissues is established through optimization of liquid chromatography conditions and experiments of a preparation method of a standard substance and a test sample; a methodology verification test shows that the established method is high in accuracy, strong in specificity and good in reproducibility, and residues of tylosin in tissues can be effectively detected.