The invention belongs to the technical field of biological materials, and discloses an antibacterial oral denture base and a preparation method thereof. The denture base is prepared from, by weight, 95-99 parts of PMMA based dental photosensitive resin, 1-2 parts of TiO2, 1-4 parts of PEEK and 0.2-0.4 part of a silane coupling agent; the antibacterial denture base is photo-cured by a 3D printer. The invention also discloses a preparation method of the antibacterial oral denture base. The antibacterial denture base has good biocompatibility, good mechanical properties, good compressive strength, bending strength, impact resistance strength, excellent antibacterial property and sustained antibacterial effect, and can solve the problem that the PMMA based resin is susceptible to microbial infection. The preparation method of the antibacterial oral denture base is simple.