The invention belongs to the technical field of preparation of lipid emulsions, and particularly relates to a nano-lipid emulsion with oxidation resistance and a preparing method thereof. According tothe preparing method, a mode of Emulsion Phrase Inversion (EPI) is adopted for preparing the nano emulsion at the normal temperature. Isopropyl myristate and lipid are selected as an oil phase, and anonionic surfactant is added into the oil phase; a cosurfactant and deionized water are mixed to serve as an aqueous phase; an antioxidant is dissolved in the aqueous phase or the oil phase accordingto the solubility of the selected antioxidant; the aqueous phase is dropwise added into the oil phase on a magnetic stirring apparatus, and then a mixture is continuously stirred for a certain periodof time to form the stable emulsion. The prepared nano-lipid emulsion has a small particle size, the particle size distribution range is narrow, and the pH stability is high.