The invention relates to a method for mass production of T cells, which comprises the following steps of:(1) wrapping the T cells in a sodium alginate rubber tube, and solidifying to form a gel tube;(2) loading the gel tube into T cell culture nests which are arranged in parallel;(3) respectively entering nutrient solution into each independent T cell culture nest to culture the T cells, and discharging metabolic products and residual nutrient solution through a nutrient solution recovery pipeline; and (4) carrying out pressure training on the T cells while culturing the T cells, so that the pressure resistance of the T cells and the survival rate after the T cells enter blood can be improved, and the T cells can be collected and applied after amplification culture. The method for mass production of the T cells can realize the mass small-yield production of the T cells, can effectively reduce the production cost of the T cells, improve the activity of the T cells and effectively meet the market demand of the T cells.