The invention discloses a trepang bait additive having an active probiotic culture medium containing traditional Chinese medicines. The active probiotic culture medium containing traditional Chinese medicines is used for performing fermentation culture on microorganisms used in trepang bait. The active probiotic culture medium containing traditional Chinese medicines comprise components of wheat bran, corn flour, brown sugar, traditional Chinese medicine components, light weight calcium carbonate and yeast cream, wherein the traditional Chinese medicine components comprise radix ginseng rubra,radix astragali, Chinese wolfberry fruits, bighead atractylodes rhizomes, poria cocos, radix angelica sinensis, licorice roots, haws, radix et rhizoma rhei, radix scutellariae, radix gentianae and honeysuckle flowers. The trepang bait additive solves the problems that in the prior art, the trepang bait additive is low in microorganism quantity, low in activity, poor in field planting capacity inintestines and the like, the microorganism quantity in the trepan bait additive can be increased, the activity of the microorganisms can be improved, and the field planting capacity in the intestinescan be improved.